| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Tarvestad, William J (20)
|
PCALTO |
51.19 |
51.23 |
0.04 |
| 2 |
Stirling, Kenneth C (24)
|
UTBYU |
50.93 |
51.38 |
0.45 |
| 3 |
Evert, Joseph J (19)
|
MDNAVY |
51.07 |
51.48 |
0.41 |
| 4 |
Essing, Matthew D (22)
|
MDTUS |
51.22 |
51.60 |
0.38 |
| 5 |
Filipovic, Aleksej A (17)
|
ILSCST |
51.55 |
51.62 |
0.07 |
| 6 |
Palmer, Thomas M (17)
|
MWLSS |
50.60 |
51.64 |
1.04 |
| 6 |
Davis, William I (21)
|
FLFLOR |
50.71 |
51.64 |
0.93 |
| 8 |
Brown, Jace C (20)
|
GUTAMU |
51.50 |
51.79 |
0.29 |
| 9 |
Harrison, Brigham B (22)
|
UTBYU |
51.41 |
51.89 |
0.48 |
| 10 |
Ledford, Mitchell P (16)
|
FLTCSC |
53.08 |
51.99 |
-1.09 |
| 11 |
Bochenski, Grant R (18)
|
ILHSC |
51.67 |
52.19 |
0.52 |
| 12 |
Tannenberger, Matthew W (18)
|
GUSSAN |
|
52.30 |
- |
| 13 |
Tang, Jonathan Y (19)
|
OKSSC |
52.62 |
52.35 |
-0.27 |
| 14 |
Forbes, Benjamin T (19)
|
ILNU |
50.80 |
52.46 |
1.66 |
| 15 |
Aulizia, Callen C (21)
|
MDNAVY |
54.03 |
52.67 |
-1.36 |
| 16 |
Reno, Seth D (18)
|
GUSSS |
51.04 |
52.70 |
1.66 |
| 17 |
Delaney, Dylan D (21)
|
WIWA |
52.92 |
53.13 |
0.21 |
| 18 |
Williams, Eli M (21)
|
MDNAVY |
52.10 |
53.41 |
1.31 |