| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hoffer, John T (12)
|
VANOVA |
24.94 |
24.09 |
-0.85 |
| 2 |
Vanas, Andrew J (12)
|
PVNCAP |
25.12 |
24.74 |
-0.38 |
| 3 |
Muminovic, Aidin A (11)
|
VANOVA |
26.76 |
25.79 |
-0.97 |
| 4 |
Chen, John D (12)
|
VANOVA |
26.52 |
25.97 |
-0.55 |
| 5 |
Franks, Benjamin D (12)
|
VANOVA |
27.75 |
26.34 |
-1.41 |
| 6 |
Martinez, Angel G (12)
|
VANOVA |
26.63 |
26.46 |
-0.17 |
| 7 |
Petkauskas, Justinas (12)
|
VANOVA |
27.46 |
27.05 |
-0.41 |
| 8 |
Farnsworth, Joshua D (12)
|
VAUN-N |
27.26 |
27.30 |
0.04 |
| 9 |
Peat, William T (11)
|
VANOVA |
28.27 |
27.43 |
-0.84 |
| 10 |
Kominski, Tyler M (11)
|
PVNCAP |
28.45 |
27.72 |
-0.73 |
| 11 |
Rivet, James B (11)
|
VANOVA |
28.11 |
28.13 |
0.02 |
| 12 |
Payne, Jonathan V (12)
|
VANOVA |
28.39 |
28.18 |
-0.21 |
| 13 |
Redfern, Cooper C (11)
|
VANOVA |
29.26 |
29.46 |
0.20 |
| 14 |
Kee, Caleb M (11)
|
PVNCAP |
30.50 |
29.50 |
-1.00 |
| 15 |
Julian, Erik P (11)
|
VANOVA |
30.04 |
30.07 |
0.03 |
| 16 |
Campello, Anderson L (11)
|
PVNCAP |
36.09 |
32.20 |
-3.89 |
|
Goetcheus, Jax A (12)
|
PVNCAP |
31.69 |
NS |
- |