| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cummins, Bodhi K (10)
|
NCUN |
33.61 |
32.91 |
-0.70 |
| 2 |
Hennis, Nolan A (10)
|
NCTYDE |
36.25 |
35.86 |
-0.39 |
| 3 |
Dickenson, Anderson D (9)
|
NCSTAR |
38.97 |
38.46 |
-0.51 |
| 4 |
Dexter, Noah E (10)
|
NCTYDE |
47.42 |
43.30 |
-4.12 |
| 5 |
Burgess-Hicks, Kal Hyun N (10)
|
NCGCY |
|
43.97 |
- |
| 6 |
Hennis, Liam R (8)
|
NCTYDE |
49.94 |
45.47 |
-4.47 |
| 7 |
Holmes, Robert C (9)
|
NCTYDE |
46.81 |
49.28 |
2.47 |
| 8 |
Snapp, Lucas A (7)
|
NCTYDE |
|
51.67 |
- |
| 9 |
Beekman, Russell J (9)
|
NCTYDE |
49.62 |
54.01 |
4.39 |
| 10 |
Bell, Tanner N (9)
|
NCTYDE |
|
59.30 |
- |
|
Cook, William H (9)
|
NCSSS |
|
DQ |
- |
|
McCall, William H (10)
|
NCSSS |
|
DQ |
- |
|
Muirhead, Ayden G (8)
|
NCSGSA |
1:06.52 |
DQ |
- |