| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Agee, Ainsley B (8)
|
VANOVA |
48.66 |
45.94 |
-2.72 |
| 2 |
Sukharevich, Diana (8)
|
VANOVA |
|
46.85 |
- |
| 3 |
Sardesai, Eva X (8)
|
VANOVA |
48.41 |
46.87 |
-1.54 |
| 4 |
Shiveley, Katherine B (8)
|
VANOVA |
|
47.49 |
- |
| 5 |
Alligood, Holly E (8)
|
VANOVA |
53.02 |
48.25 |
-4.77 |
| 6 |
Pyle, Madelyn M (8)
|
VANOVA |
52.51 |
49.16 |
-3.35 |
| 7 |
Sady, Teagan G (8)
|
VAPSDN |
51.49 |
49.87 |
-1.62 |
| 8 |
Price, Lindsey E (8)
|
VANOVA |
51.76 |
53.02 |
1.26 |
| 9 |
Dennis, Genevieve L (8)
|
VANOVA |
|
54.05 |
- |
| 10 |
Rubin, Sofiamari (8)
|
VANOVA |
|
54.24 |
- |
| 11 |
Hendrick, Abigail N (8)
|
VANOVA |
|
56.86 |
- |
| 12 |
Yu, Chen Rosemary (8)
|
VANOVA |
|
56.89 |
- |
| 13 |
Sexstone, Charlotte N (8)
|
VANOVA |
|
58.73 |
- |
| 14 |
Cantrell, Emma K (8)
|
VANOVA |
|
59.22 |
- |
| 15 |
Dorfman, Adelaide A (8)
|
VANOVA |
1:05.36 |
1:01.67 |
-3.69 |
| 16 |
Patterson, London (8)
|
VANOVA |
|
1:02.14 |
- |
| 17 |
Clarke, Beatrice B (8)
|
VANOVA |
|
1:02.81 |
- |
| 18 |
Johnstone, Camille T (8)
|
VANOVA |
|
1:04.75 |
- |
| 19 |
Watts, Riley D (8)
|
VAPSDN |
|
1:21.99 |
- |
|
Liu, Eva C (8)
|
VANOVA |
|
DQ |
- |
|
Hopkins, Acree E (8)
|
VANOVA |
|
DQ |
- |