| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kantner, Emily R (15)
|
VANOVA |
2:38.99 |
2:44.33 |
5.34 |
| 2 |
Nelson, Brooke M (17)
|
VANOVA |
3:01.54 |
2:54.00 |
-7.54 |
| 3 |
Dillman, Shannah R (17)
|
VANOVA |
2:53.03 |
2:57.57 |
4.54 |
| 4 |
Connor, Lillie D (15)
|
VANOVA |
3:06.84 |
3:03.16 |
-3.68 |
| 5 |
Witdoeckt, Allison G (15)
|
VANOVA |
3:25.72 |
3:10.49 |
-15.23 |
| 6 |
Bauer, Cassandra G (15)
|
VAHNVR |
3:14.10 |
3:14.34 |
0.24 |
| 7 |
Allison, Emma B (15)
|
VAHNVR |
|
3:22.93 |
- |
| 8 |
Crampton, Isabella E (17)
|
VATSU |
3:36.37 |
3:32.01 |
-4.36 |
| 9 |
Labrusciano, Anna F (15)
|
VATSU |
3:37.36 |
3:38.02 |
0.66 |
| 10 |
Bain, Mattie J (16)
|
VATSU |
3:45.87 |
3:44.19 |
-1.68 |