| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bud, Ellie S (10)
|
MRLIAC |
36.21 |
35.28 |
-0.93 |
| 1 |
Fuccillo, Ella R (10)
|
MRWISC |
42.20 |
44.77 |
2.57 |
| 2 |
Nguyen, Olivia B (10)
|
MRSSC |
38.84 |
36.07 |
-2.77 |
| 3 |
Petrovic, Branka D (10)
|
MRTVSC |
38.18 |
37.20 |
-0.98 |
| 4 |
Lowery, Doris M (10)
|
MRLIAC |
40.25 |
39.09 |
-1.16 |
| 5 |
Augustin, Jaclyn E (10)
|
MRFA |
40.69 |
39.52 |
-1.17 |
| 6 |
O'Shaughnessy, Kaitlyn F (10)
|
MRCSDC |
42.19 |
39.75 |
-2.44 |
| 7 |
Boyon, Lily A (10)
|
MRFA |
38.73 |
39.76 |
1.03 |
| 8 |
O'Brien, Katherine S (10)
|
MRLIAC |
39.23 |
39.95 |
0.72 |
| 9 |
Hu, Allison (10)
|
MRLIAC |
40.08 |
41.88 |
1.80 |