| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mullen, Violet E (11)
|
PVNCAP |
35.13 |
33.78 |
-1.35 |
| 2 |
Bruning, Charlotte G (11)
|
VANOVA |
34.60 |
34.08 |
-0.52 |
| 3 |
Hersh, Kallie M (11)
|
PVNCAP |
35.39 |
34.82 |
-0.57 |
| 4 |
Hatfield, Sophia L (11)
|
PVNCAP |
36.03 |
35.00 |
-1.03 |
| 5 |
Simons, Audrey K (11)
|
MRLIAC |
36.34 |
35.56 |
-0.78 |
| 6 |
Ehrensberger, Margaret C (11)
|
VANOVA |
36.80 |
36.33 |
-0.47 |
| 7 |
Paradise, Therese Y (11)
|
PVNCAP |
37.61 |
36.77 |
-0.84 |
| 8 |
Carvalho, Julia K (11)
|
VANOVA |
38.41 |
37.39 |
-1.02 |
| 9 |
Grossgold, Clara L (11)
|
VATIDE |
39.70 |
38.35 |
-1.35 |
| 10 |
Souffront, Olivia R (11)
|
MRLIAC |
40.21 |
41.27 |
1.06 |
| 11 |
Darcy, Cate Q (11)
|
MRLIAC |
43.48 |
41.58 |
-1.90 |
| 12 |
Perkins, Kielei L (11)
|
VATIDE |
46.30 |
44.18 |
-2.12 |
| 13 |
Hartley, Sophie A (11)
|
VATIDE |
46.93 |
45.44 |
-1.49 |
| 14 |
Patterson, Emma M (11)
|
VATIDE |
47.78 |
45.85 |
-1.93 |
|
Alligood, Holly E (11)
|
VANOVA |
39.76 |
NS |
- |