| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Marshall, Vivian M (12)
|
PVNCAP |
26.21 |
25.99 |
-0.22 |
| 2 |
Morgan, Alexandra M (12)
|
PVNCAP |
26.49 |
26.14 |
-0.35 |
| 3 |
Knight, Josephine (12)
|
VATIDE |
26.84 |
26.16 |
-0.68 |
| 4 |
Swann, Drew M (12)
|
VATIDE |
26.53 |
26.24 |
-0.29 |
| 5 |
Dufault, Audrey A (12)
|
PVNCAP |
26.64 |
26.46 |
-0.18 |
| 6 |
Straesser, Mae T (12)
|
MRLIAC |
26.61 |
26.60 |
-0.01 |
| 7 |
Moore, Caitlyn N (12)
|
VANOVA |
26.70 |
26.83 |
0.13 |
| 8 |
Francis, Nora M (12)
|
VANOVA |
28.13 |
27.06 |
-1.07 |
| 9 |
Lee, Lauren J (12)
|
PVNCAP |
27.43 |
27.11 |
-0.32 |
| 10 |
Shean, May E (12)
|
VATIDE |
28.10 |
27.37 |
-0.73 |
| 11 |
Bouchard, Gabrielle M (12)
|
VANOVA |
28.40 |
27.56 |
-0.84 |
| 12 |
Phadtare, Ashlesha A (12)
|
VANOVA |
27.67 |
27.66 |
-0.01 |
| 13 |
Lowery, Doris M (12)
|
MRLIAC |
28.31 |
28.10 |
-0.21 |
| 14 |
Piper, Vanessa L (12)
|
VATIDE |
28.13 |
28.71 |
0.58 |
| 15 |
Hu, Allison (12)
|
MRLIAC |
30.33 |
29.60 |
-0.73 |