| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Moore, Coyle F (12)
|
VATIDE |
24.01 |
24.03 |
0.02 |
| 2 |
O'Neill, Connor F (12)
|
PVNCAP |
25.11 |
24.49 |
-0.62 |
| 3 |
Yao, Maxwell (12)
|
MRLIAC |
26.00 |
25.32 |
-0.68 |
| 4 |
Luo, Howard H (12)
|
VANOVA |
25.83 |
25.61 |
-0.22 |
| 5 |
Miklos, Owen T (12)
|
VANOVA |
27.08 |
25.95 |
-1.13 |
| 6 |
Hughes, Cayden M (12)
|
PVNCAP |
26.27 |
25.97 |
-0.30 |
| 7 |
Li, Eric (12)
|
MRLIAC |
25.70 |
26.01 |
0.31 |
| 8 |
Strickland, Owen M (12)
|
PVNCAP |
25.98 |
26.35 |
0.37 |
| 9 |
Guo, Sean Z (12)
|
PVNCAP |
26.73 |
26.56 |
-0.17 |
| 10 |
Van Deren, Jackson R (12)
|
VANOVA |
26.91 |
26.78 |
-0.13 |
| 11 |
Sardesai, Nolan A (12)
|
VANOVA |
27.44 |
26.83 |
-0.61 |
| 12 |
Alcox, Preston T (12)
|
VATIDE |
27.60 |
27.35 |
-0.25 |
| 13 |
Radoslovich, Steven A (12)
|
MRLIAC |
28.27 |
27.45 |
-0.82 |
| 14 |
Peng, Warren (12)
|
MRLIAC |
29.48 |
28.37 |
-1.11 |
| 15 |
Sanness, Lukas T (12)
|
VATIDE |
30.45 |
29.94 |
-0.51 |
| 16 |
Jacobs, Ezekiel T (12)
|
VATIDE |
31.53 |
30.14 |
-1.39 |