| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
DeGuenther, Emory B (14)
|
VANOVA |
56.31 |
56.93 |
0.62 |
| 2 |
Sagle, Alyssa K (14)
|
PVNCAP |
56.46 |
58.03 |
1.57 |
| 3 |
Kilty, Caroline R (14)
|
PVNCAP |
1:02.00 |
1:00.26 |
-1.74 |
| 4 |
Blanco, Karina I (14)
|
VANOVA |
1:01.38 |
1:00.60 |
-0.78 |
| 5 |
Carvalho, Gabriela (14)
|
VANOVA |
1:00.11 |
1:00.61 |
0.50 |
| 6 |
Clattenburg, Hannah C (14)
|
PVNCAP |
1:02.21 |
1:00.84 |
-1.37 |
| 7 |
Wright, Margaret Ann E (14)
|
VATIDE |
1:00.47 |
1:00.98 |
0.51 |
| 8 |
Heath, Briana L (14)
|
PVNCAP |
1:02.11 |
1:00.99 |
-1.12 |
| 9 |
Spooner, Olivia E (14)
|
VANOVA |
1:01.42 |
1:01.49 |
0.07 |
| 10 |
Marchione, Maris J (14)
|
VATIDE |
1:02.35 |
1:03.05 |
0.70 |
| 11 |
Brecke, Avery M (14)
|
VATIDE |
1:07.80 |
1:08.49 |
0.69 |
| 12 |
Eberle, Layla G (14)
|
VATIDE |
1:05.38 |
1:08.57 |
3.19 |
|
Lyons, Sophia J (13)
|
MRLIAC |
|
1:04.56 |
- |
|
Davidoff, Sadie C (13)
|
MRLIAC |
|
1:05.90 |
- |