| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Brenner, Ellie M (10)
|
PVNCAP |
27.45 |
28.24 |
0.79 |
| 2 |
Marm, Reagan E (10)
|
PVNCAP |
29.69 |
29.18 |
-0.51 |
| 3 |
Franks, Keeley J (10)
|
PVNCAP |
30.52 |
29.56 |
-0.96 |
| 4 |
Bruning, Charlotte G (10)
|
VANOVA |
30.79 |
29.58 |
-1.21 |
| 5 |
Koroma, Camille Y (10)
|
PVNCAP |
30.92 |
29.77 |
-1.15 |
| 6 |
Kitlowski, Kara O (10)
|
MRLIAC |
30.54 |
29.81 |
-0.73 |
| 7 |
Ehrensberger, Margaret C (10)
|
VANOVA |
31.40 |
30.50 |
-0.90 |
| 8 |
Carvalho, Julia K (10)
|
VANOVA |
32.11 |
31.68 |
-0.43 |
| 9 |
Bailey, Mackenzie S (10)
|
VANOVA |
31.62 |
31.70 |
0.08 |
| 10 |
Li, Chelsea T (10)
|
VATIDE |
33.85 |
31.82 |
-2.03 |
| 11 |
Bringle, Ashley (10)
|
MRLIAC |
32.28 |
32.07 |
-0.21 |
| 12 |
Johnson, Chloe M (10)
|
VATIDE |
35.09 |
32.11 |
-2.98 |
| 13 |
Bregman, Mollie E (10)
|
MRLIAC |
34.75 |
33.60 |
-1.15 |
| 14 |
Perkins, Kielei L (10)
|
VATIDE |
32.48 |
34.30 |
1.82 |
| 15 |
Carroll, Emma C (10)
|
VATIDE |
|
38.56 |
- |