| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Karasiewicz, Hayden A (9)
|
MDBAY |
46.54 |
43.79 |
-2.75 |
| 2 |
Xu, Miranda (10)
|
PVMAC |
47.22 |
45.42 |
-1.80 |
| 3 |
Sun, Madeleine W (10)
|
PVMAC |
46.19 |
45.74 |
-0.45 |
| 4 |
Park, Lillian S (10)
|
PVMAC |
48.56 |
46.86 |
-1.70 |
| 5 |
Kang, Inah I (9)
|
PVMAC |
46.67 |
47.31 |
0.64 |
| 6 |
Hill, Priya S (9)
|
PVASTS |
50.56 |
50.65 |
0.09 |
| 7 |
Kuhn, Charlotte (10)
|
MDBAY |
56.37 |
53.59 |
-2.78 |
| 8 |
Lionel, Caidyn (10)
|
MDBAY |
1:07.59 |
56.44 |
-11.15 |
| 9 |
Lewis, Reagan Q (10)
|
MDBAY |
1:01.84 |
59.69 |
-2.15 |
| 10 |
Benjamin, Samantha (10)
|
MDBAY |
1:06.36 |
1:02.69 |
-3.67 |
| 11 |
Garcia, Betsy (9)
|
PVASTS |
|
1:09.29 |
- |
| 12 |
Bradley, Eliana (7)
|
MDBAY |
|
1:11.52 |
- |
| 13 |
Bottner, Faith (10)
|
MDBAY |
1:22.90 |
1:11.96 |
-10.94 |
| 14 |
Berger, Dahlia (8)
|
MDBAY |
|
1:13.84 |
- |
| 15 |
Koontz, Lily A (8)
|
MDBAY |
1:15.51 |
1:14.85 |
-0.66 |
| 16 |
Gonzalez, Ruby M (9)
|
PVASTS |
|
1:27.34 |
- |
| 17 |
Wenzel, Grace (9)
|
MDBAY |
|
1:31.80 |
- |
|
Kuhn, Caroline (9)
|
MDBAY |
|
DQ |
- |
|
Kennedy, Madeline (10)
|
MDBAY |
|
DQ |
- |
|
Sanders, Camille (7)
|
PVASTS |
|
DQ |
- |
|
Sampson-Walawender, Ella M (9)
|
PVASTS |
|
DQ |
- |
|
Piper, Mikhaila E (6)
|
PVASTS |
|
DQ |
- |
|
Owusu, Amina M (10)
|
PVASTS |
|
DQ |
- |
|
Pierola, Alessandra A (10)
|
PVFAST |
1:02.09 |
NS |
- |