| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Harbour, Brennan P (10)
|
VARACE |
51.17 |
46.89 |
-4.28 |
| 2 |
Dugger, Sophia A (9)
|
VAUN |
52.33 |
48.41 |
-3.92 |
| 3 |
Perko, Lillian J (10)
|
VASRVA |
|
49.18 |
- |
| 4 |
Haney, Sarah E (10)
|
VASRVA |
|
49.97 |
- |
| 5 |
Bonyadlou, Taraneh B (9)
|
VA757 |
54.11 |
50.12 |
-3.99 |
| 6 |
Polo Reasor, Mia C (9)
|
VAUN |
1:01.59 |
51.04 |
-10.55 |
| 7 |
Cleary, Peyton M (10)
|
VA757 |
|
51.80 |
- |
| 8 |
Daniel, Campbell S (9)
|
VA757 |
1:07.47 |
51.91 |
-15.56 |
| 9 |
Quinn, Brooke A (9)
|
VA757 |
|
51.96 |
- |
| 10 |
Popelars, Zoe G (10)
|
VA757 |
1:00.69 |
52.75 |
-7.94 |
| 11 |
Heale, Annie C (9)
|
VASMAC |
|
53.27 |
- |
| 12 |
Williamson, Addison O (10)
|
VAUN |
59.65 |
53.29 |
-6.36 |
| 13 |
Fly, Kayla G (9)
|
VA757 |
1:12.55 |
54.42 |
-18.13 |
| 14 |
Kirkpatrick, Delaney P (10)
|
VA757 |
58.25 |
57.40 |
-0.85 |
| 15 |
Wells, Kenley A (9)
|
VAUN |
59.28 |
57.75 |
-1.53 |
| 16 |
Richardi, Cameron M (9)
|
VA757 |
|
58.99 |
- |
| 17 |
Brodie, Chelsea A (9)
|
VA757 |
|
59.37 |
- |
| 18 |
White, Alyssa A (10)
|
VA757 |
|
1:04.92 |
- |
| 19 |
Ananthram, Seva J (9)
|
VA757 |
|
1:05.55 |
- |
|
Younica, Eleanor A (9)
|
VA757 |
|
DQ |
- |
|
Fontenot, Samantha G (10)
|
VASRVA |
|
DQ |
- |
|
Ericsson, Brooke A (9)
|
VA757 |
|
DQ |
- |
|
McKay, Frances E (10)
|
VAUN |
55.38 |
DQ |
- |