| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mizuba, Hannah R (12)
|
VASFY |
35.90 |
35.22 |
-0.68 |
| 2 |
Heyward, Addison E (12)
|
VA757 |
43.03 |
35.40 |
-7.63 |
| 3 |
Bing, Rachael C (12)
|
VAGATR |
35.52 |
35.57 |
0.05 |
| 4 |
Gorby, Tessa L (12)
|
VAGATR |
42.59 |
37.52 |
-5.07 |
| 5 |
Lorack, Isabella G (11)
|
VA757 |
38.96 |
37.59 |
-1.37 |
| 6 |
Keller, Madison G (12)
|
VAGATR |
39.09 |
39.31 |
0.22 |
| 7 |
Couric, Rebecca B (12)
|
VAGATR |
39.59 |
39.74 |
0.15 |
| 8 |
Carter, Emmi D (11)
|
VACCA |
44.95 |
40.35 |
-4.60 |
| 9 |
Spurlock, Avery M (11)
|
VACCA |
44.67 |
40.52 |
-4.15 |
| 10 |
Conforti-Johnson, Sophia K (12)
|
VACCA |
|
40.82 |
- |
| 11 |
Belde, Lily A (12)
|
VARACE |
45.77 |
40.83 |
-4.94 |
| 12 |
Viar, Olivia B (11)
|
VACCA |
45.54 |
41.30 |
-4.24 |
| 13 |
Hoang, Angelina (11)
|
VAACAC |
|
41.86 |
- |
| 14 |
Jones, Cary E (11)
|
VASFY |
46.25 |
43.78 |
-2.47 |
| 15 |
O'Neill, Miranda B (12)
|
VAGATR |
|
44.76 |
- |
| 16 |
Cleary, Peyton M (11)
|
VA757 |
49.64 |
46.05 |
-3.59 |
| 17 |
Brisson, Bethany M (12)
|
VA757 |
48.50 |
47.85 |
-0.65 |
| 18 |
Shaltayeva, Victoria (11)
|
VASEVA |
48.11 |
48.50 |
0.39 |
| 19 |
Haas, Natalie R (11)
|
VA757 |
56.00 |
49.11 |
-6.89 |
| 20 |
White, Alyssa A (11)
|
VA757 |
59.65 |
49.60 |
-10.05 |