| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Medei, Emma L (8)
|
VANOVA |
44.53 |
43.35 |
-1.18 |
| 2 |
Meyer, Amelie G (8)
|
VAACAC |
45.98 |
44.81 |
-1.17 |
| 3 |
Eubank, Mia L (8)
|
VANOVA |
45.58 |
44.85 |
-0.73 |
| 4 |
Rajagopalan, Tilda (8)
|
VAACAC |
44.59 |
45.91 |
1.32 |
| 5 |
Harris, Savannah J (7)
|
VANOVA |
|
50.75 |
- |
| 6 |
Holland, Samantha C (8)
|
VATSU |
57.60 |
55.74 |
-1.86 |
| 7 |
Richardson, Reese G (8)
|
VANOVA |
56.18 |
56.41 |
0.23 |
| 8 |
Makris, Nicole V (8)
|
VANOVA |
1:06.12 |
57.06 |
-9.06 |
| 9 |
Wells, Kenley A (8)
|
VADC |
1:13.82 |
1:02.04 |
-11.78 |
| 10 |
Dunford, Mackenzie N (8)
|
VANOVA |
|
1:04.61 |
- |
| 11 |
Franchok, Caroline E (8)
|
VANOVA |
|
1:10.39 |
- |
| 12 |
Clark, Madison T (8)
|
VABASS |
|
1:11.40 |
- |
|
Mears, Vivian L (7)
|
VADC |
|
DQ |
- |
|
Hines, Audra J (7)
|
VABASS |
|
NS |
- |
|
Williams, Jaquelin Holt A (8)
|
VADC |
|
NS |
- |