| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kolb, Diana E (19)
|
GUAMBU |
2:35.25 |
2:35.47 |
0.22 |
| 2 |
Horne, Jamie A (17)
|
LAYNWL |
2:59.29 |
3:00.27 |
0.98 |
| 3 |
Courtney, Natalie L (15)
|
GUAMBU |
3:26.43 |
3:03.81 |
-22.62 |
| 4 |
McKee, Lily P (16)
|
NTLIFE |
|
3:10.46 |
- |
| 5 |
Nieberding, Vivyan E (15)
|
GUAMBU |
|
3:15.81 |
- |
| 6 |
Haase, Liberty E (15)
|
LAYNWL |
3:11.85 |
3:16.57 |
4.72 |
| 7 |
Armstrong, Jordan D (17)
|
GUAMBU |
2:50.29 |
3:17.54 |
27.25 |
| 8 |
Deshotel, Madison N (16)
|
NTTRAC |
|
3:18.63 |
- |
| 9 |
Romanski, Isabella G (15)
|
LAYNWL |
|
3:47.59 |
- |
|
Jackson, Abigail E (16)
|
LAYNWL |
2:56.13 |
NS |
- |