| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Frost, Hope C (12)
|
VADIG |
31.65 |
31.92 |
0.27 |
| 2 |
Timberg, Emma C (12)
|
VA757 |
|
37.61 |
- |
| 3 |
Martin, Avery L (12)
|
VA757 |
|
38.22 |
- |
| 4 |
Shearer, Jacquelyn M (11)
|
VA757 |
41.49 |
38.95 |
-2.54 |
| 5 |
Strite, Carter E (11)
|
VARACE |
41.98 |
38.98 |
-3.00 |
| 6 |
Lohr, Faith M (12)
|
VA757 |
|
40.01 |
- |
| 7 |
Olsen, Sarah A (11)
|
VA757 |
|
40.62 |
- |
| 8 |
Dodson, Sadie (11)
|
VA757 |
|
45.82 |
- |
| 9 |
Masengale, Charlotte M (12)
|
VA757 |
|
46.76 |
- |
| 10 |
Timberg, Mia A (11)
|
VA757 |
|
46.86 |
- |
| 11 |
Monestine, Ymanee R (12)
|
VAUN-Q |
|
47.15 |
- |
| 12 |
Heyward, Alexandra P (11)
|
VAUN75 |
|
48.99 |
- |
| 13 |
Wester, Madison E (11)
|
VA757 |
|
50.55 |
- |
|
Ashby, Leah P (12)
|
VADIG |
36.68 |
NS |
- |