| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Frost, Hope C (12)
|
VADIG |
38.15 |
35.28 |
-2.87 |
| 2 |
Martin, Avery L (12)
|
VA757 |
|
40.64 |
- |
| 3 |
Strite, Carter E (11)
|
VARACE |
43.28 |
40.90 |
-2.38 |
| 4 |
Shearer, Jacquelyn M (11)
|
VA757 |
41.57 |
42.13 |
0.56 |
| 5 |
Masengale, Charlotte M (12)
|
VA757 |
|
43.31 |
- |
| 6 |
Lohr, Faith M (12)
|
VA757 |
|
43.63 |
- |
| 7 |
Timberg, Mia A (11)
|
VA757 |
|
44.27 |
- |
| 8 |
Olsen, Sarah A (11)
|
VA757 |
|
46.21 |
- |
| 9 |
Monestine, Ymanee R (12)
|
VAUN-Q |
|
48.08 |
- |
| 10 |
Dodson, Sadie (11)
|
VA757 |
|
48.26 |
- |
| 11 |
Heyward, Alexandra P (11)
|
VAUN75 |
|
53.05 |
- |
| 12 |
Wester, Madison E (11)
|
VA757 |
|
53.71 |
- |
|
Timberg, Emma C (12)
|
VA757 |
|
DQ |
- |
|
Ashby, Leah P (12)
|
VADIG |
34.58 |
NS |
- |