| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rhodes, Cameron M (16)
|
VANOVA |
22.82 |
22.89 |
0.07 |
| 2 |
Jena, Amish (16)
|
VANOVA |
23.97 |
23.18 |
-0.79 |
| 3 |
Lifsey, Judson C (18)
|
VANOVA |
22.60 |
23.27 |
0.67 |
| 4 |
Balk, Jordan A (16)
|
VANOVA |
23.07 |
23.36 |
0.29 |
| 5 |
Stallard, Adam H (17)
|
VANOVA |
24.23 |
23.89 |
-0.34 |
| 6 |
VanLesser, Brogan Z (15)
|
VANOVA |
24.66 |
24.12 |
-0.54 |
| 7 |
Panos, Gregory N (17)
|
VANOVA |
23.80 |
24.37 |
0.57 |
| 8 |
Schwabe, John W (15)
|
VANOVA |
24.71 |
24.55 |
-0.16 |
| 9 |
Gantz, Shane D (17)
|
VANOVA |
23.44 |
24.59 |
1.15 |
| 10 |
Ehrensberger, Benedict M (15)
|
VANOVA |
25.32 |
24.70 |
-0.62 |
| 11 |
Ng, Zachary K (15)
|
VANOVA |
25.57 |
25.09 |
-0.48 |
| 12 |
Gupton, Thomas E (15)
|
VANOVA |
26.91 |
25.30 |
-1.61 |
| 13 |
Whitesell, Remington A (15)
|
VANOVA |
25.98 |
25.63 |
-0.35 |
| 14 |
Holmes, James E (16)
|
VANOVA |
25.07 |
25.74 |
0.67 |
| 15 |
Maiden, Mark H (15)
|
VANOVA |
25.46 |
25.80 |
0.34 |
| 16 |
Boucher, Griffin X (17)
|
VANOVA |
25.27 |
25.99 |
0.72 |
| 17 |
Li, Yuxuan (15)
|
VANOVA |
26.68 |
26.65 |
-0.03 |
| 18 |
Hurlbert, Jack (16)
|
VANOVA |
|
26.68 |
- |
| 19 |
Scharpf, Ryan M (15)
|
VANOVA |
27.32 |
27.44 |
0.12 |