| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hollender, Brighton C (7)
|
VAQSTS |
39.24 |
38.42 |
-0.82 |
| 2 |
Wirt, Caroline F (7)
|
VAPSDN |
39.61 |
39.95 |
0.34 |
| 3 |
Griffin, Alexis G (7)
|
VAQSTS |
45.47 |
46.45 |
0.98 |
| 4 |
Handral, Dwani P (7)
|
VANOVA |
49.72 |
48.42 |
-1.30 |
| 5 |
Gutierrez, Julianna M (7)
|
VANOVA |
45.33 |
49.00 |
3.67 |
| 6 |
Hromyak, Alexis (7)
|
VANOVA |
|
50.23 |
- |
| 7 |
Kulkarni, Aranyaa (7)
|
VACA-Y |
53.13 |
54.49 |
1.36 |
| 8 |
Sunkavalli, Chaithra (7)
|
VANOVA |
49.78 |
55.53 |
5.75 |
| 9 |
Perea Romero, Maricielo R (7)
|
VANOVA |
53.35 |
57.45 |
4.10 |
| 10 |
Murphy, Serena C (7)
|
VANOVA |
|
1:00.20 |
- |
| 11 |
Allen, Josephine A (7)
|
VANOVA |
59.11 |
1:01.48 |
2.37 |
| 12 |
Green, Stella D (7)
|
VANOVA |
58.65 |
1:01.93 |
3.28 |
| 13 |
Ravuru, Aadhya (7)
|
VANOVA |
1:03.17 |
1:03.82 |
0.65 |
| 14 |
Winslow, Eliza B (7)
|
VANOVA |
1:05.96 |
1:10.10 |
4.14 |