| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mendez-Guzman, Doreli N (13)
|
VAGATR |
26.38 |
26.56 |
0.18 |
| 2 |
Pillion, Abby R (14)
|
VAGATR |
28.18 |
28.09 |
-0.09 |
| 2 |
Wang, Amy C (13)
|
VAGATR |
28.28 |
28.09 |
-0.19 |
| 4 |
Wright, Alexia R (14)
|
VAGATR |
29.18 |
28.14 |
-1.04 |
| 5 |
Deeble, Zoe E (13)
|
VAGATR |
29.11 |
28.76 |
-0.35 |
| 6 |
Williams, Annalise M (13)
|
PVRY |
28.75 |
29.47 |
0.72 |
| 7 |
Kullander, Abigail L (13)
|
VAGATR |
31.30 |
29.77 |
-1.53 |
| 8 |
Green, Mackenzie B (14)
|
VANOVA |
30.08 |
30.31 |
0.23 |
| 9 |
Pietrowski, Anja J (14)
|
VASMAC |
31.94 |
31.48 |
-0.46 |
| 10 |
Dimaiuta, Anna J (13)
|
PVRY |
42.09 |
38.64 |
-3.45 |
| 11 |
Gutterman, Rose (13)
|
VAACST |
|
41.22 |
- |
| 12 |
Farley, Sydney (13)
|
VAACST |
|
42.99 |
- |
|
Malone, Jane E (14)
|
VAACST |
|
NS |
- |
|
Sivak, Morgan E (14)
|
PVRY |
29.84 |
NS |
- |