| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Er, Melissa (9)
|
NIUN |
41.91 |
35.01 |
-6.90 |
| 2 |
Wamsley, Sarah E (9)
|
NIVELO |
38.79 |
35.84 |
-2.95 |
| 3 |
Gachette, Marisa B (9)
|
NIVELO |
41.78 |
36.07 |
-5.71 |
| 4 |
Philbrick, Mylie E (9)
|
NIVELO |
42.58 |
38.21 |
-4.37 |
| 5 |
Maynard, Isabella M (9)
|
NIWWSC |
37.00 |
38.43 |
1.43 |
| 6 |
Barillari, Emma (9)
|
NIVELO |
38.98 |
39.71 |
0.73 |
| 7 |
King, Skylar L (9)
|
NIEAST |
40.85 |
39.77 |
-1.08 |
| 8 |
Peiper, Reagan S (9)
|
NIBAAC |
41.58 |
40.10 |
-1.48 |
| 9 |
Reukauf, Anna R (9)
|
NISTAR |
43.82 |
41.64 |
-2.18 |
| 10 |
Strittmatter, Amelia C (9)
|
NISTAR |
42.01 |
41.99 |
-0.02 |
| 11 |
Weisenburg, Brylee M (9)
|
NISTAR |
46.45 |
44.01 |
-2.44 |
| 12 |
Kauffman, Faith M (9)
|
NISTAR |
48.40 |
46.08 |
-2.32 |
| 13 |
Tothero, Madeleine S (9)
|
NISTAR |
56.58 |
52.15 |
-4.43 |
| 14 |
Barrera-Hagner, Ananya S (9)
|
NIEAST |
57.86 |
52.35 |
-5.51 |
| 15 |
Stevens, Eleanor R (9)
|
NISTAR |
|
1:02.10 |
- |