| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Edwards, Victoria R (16)
|
STTXLA |
59.98 |
59.87 |
-0.11 |
| 2 |
Hinds, Natalie A (21)
|
WTCOM |
59.85 |
1:00.07 |
0.22 |
| 3 |
Magnuson, Hannah B (19)
|
KYUOFL |
1:01.23 |
1:00.82 |
-0.41 |
| 4 |
Ama, Julia G (20)
|
PCSTAN |
1:01.20 |
1:01.16 |
-0.04 |
| 5 |
Koletic, Ashlyn M (21)
|
NCUN-0 |
1:02.69 |
1:01.27 |
-1.42 |
| 6 |
Zimmerman, Madeline M (19)
|
CTUN-0 |
1:01.21 |
1:01.41 |
0.20 |
| 7 |
Okada, Amy A (17)
|
CARYL |
1:01.19 |
1:01.54 |
0.35 |
| 8 |
Labonge, Natalie J (19)
|
NCUN-0 |
1:01.51 |
1:01.78 |
0.27 |
| 9 |
Yambor-Maul, Alyssa M (20)
|
FLATAC |
1:03.00 |
1:01.79 |
-1.21 |
| 10 |
Barker, Olivia K (20)
|
INIU |
1:02.71 |
1:02.13 |
-0.58 |
| 11 |
Norman, Laura R (19)
|
GUAGS |
1:02.24 |
1:02.16 |
-0.08 |
| 12 |
Kuhn, Sierra L (21)
|
FLUN-0 |
1:02.50 |
1:02.19 |
-0.31 |
| 13 |
Quinn, Anna E (19)
|
CACSSC |
1:01.96 |
1:02.21 |
0.25 |
| 14 |
Wong, Brynne A (19)
|
STTXLA |
1:01.06 |
1:02.26 |
1.20 |
| 15 |
Acker, Rachael C (21)
|
PCCAL |
1:02.00 |
1:02.59 |
0.59 |
| 16 |
Stone, Jamie M (16)
|
ORMHA |
1:02.67 |
1:02.61 |
-0.06 |
| 17 |
Siebenaller, Samantha A (17)
|
WIMFSC |
1:04.02 |
1:03.28 |
-0.74 |
| 18 |
Hayden, Rachel E (19)
|
MVUMIZ |
1:04.80 |
1:03.48 |
-1.32 |
| 19 |
Beil, Mallory J (15)
|
OHCLPR |
1:04.00 |
1:04.60 |
0.60 |
| 20 |
Klotz, Stephanie L (21)
|
SNSMST |
1:05.50 |
1:05.02 |
-0.48 |