| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ward, Andrea M (22)
|
CAUCSB |
59.85 |
59.85 |
0.00 |
| 2 |
Bestor, Megan C (19)
|
GASA |
1:02.49 |
1:00.73 |
-1.76 |
| 3 |
Metzger-Seymour, Erin M (19)
|
MVUMIZ |
1:01.20 |
1:01.02 |
-0.18 |
| 4 |
Seiler, Erin E (21)
|
LEFAST |
1:00.77 |
1:01.28 |
0.51 |
| 5 |
Koletic, Ashlyn M (21)
|
NCUN-0 |
1:01.27 |
1:01.83 |
0.56 |
| 6 |
Norman, Laura R (19)
|
GUAGS |
1:02.20 |
1:01.88 |
-0.32 |
| 7 |
Lundstrom, Heather M (20)
|
SETNAQ |
1:01.00 |
1:02.07 |
1.07 |
| 8 |
Sowinski, Katelyn E (20)
|
MAUN-0 |
1:01.91 |
1:02.40 |
0.49 |
| 9 |
Richter, Abigail N (16)
|
CABCH |
1:03.27 |
1:02.51 |
-0.76 |
| 10 |
Kastigar, Hannah J (19)
|
SDASC |
1:02.65 |
1:02.57 |
-0.08 |
| 11 |
Slabe, Emily K (19)
|
NCNCAC |
1:02.50 |
1:03.53 |
1.03 |
| 12 |
McHugh, Cameron F (19)
|
AZFORD |
1:02.00 |
1:03.67 |
1.67 |