| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Comerford, Mallory E (17)
|
KYUN-0 |
26.50 |
25.80 |
-0.70 |
| 2 |
Gruber, Margaret A (19)
|
VAHOKI |
26.88 |
26.15 |
-0.73 |
| 3 |
Berg, Marissa K (19)
|
WIWA |
26.21 |
26.19 |
-0.02 |
| 4 |
Malone, Kristin T (19)
|
GUUN-0 |
26.30 |
26.24 |
-0.06 |
| 5 |
Liu, Kenisha R (16)
|
CABREA |
26.79 |
26.27 |
-0.52 |
| 6 |
Oliver, Meredith M (21)
|
GUAGS |
26.80 |
26.35 |
-0.45 |
| 7 |
Harper, Holland J (21)
|
VAHOKI |
26.89 |
26.37 |
-0.52 |
| 7 |
Sougstad, Emma L (20)
|
IAIOWA |
26.26 |
26.37 |
0.11 |
| 9 |
Bailey, Chelsea L (22)
|
SISDSU |
26.00 |
26.38 |
0.38 |
| 10 |
Engel, Lindsey E (19)
|
PCSTAN |
26.77 |
26.40 |
-0.37 |
| 11 |
Pammett, Cynthia A (21)
|
PCWCAB |
27.00 |
26.45 |
-0.55 |
| 12 |
Nordmann, Lucie M (15)
|
GUTWST |
26.57 |
26.48 |
-0.09 |
| 13 |
Wong, Brynne A (19)
|
STTXLA |
26.80 |
26.51 |
-0.29 |
| 14 |
Schneider, Mary E (19)
|
STTXLA |
25.91 |
26.53 |
0.62 |
| 15 |
Madden, Paige C (16)
|
SECMSA |
26.86 |
26.65 |
-0.21 |
| 16 |
Galletti, Catherine A (20)
|
INIA |
26.33 |
26.66 |
0.33 |
| 17 |
Algee, Angela J (20)
|
FGCANE |
26.40 |
26.73 |
0.33 |
| 18 |
Sinatro, Haley M (21)
|
MAUN-0 |
26.60 |
26.85 |
0.25 |
| 19 |
Saurborn, Kathryn R (24)
|
NCUN-0 |
25.97 |
27.19 |
1.22 |
| 20 |
Chamberlain, Marie C (19)
|
INIU |
27.48 |
27.45 |
-0.03 |