| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Williams, Trenton F (21)
|
PCCAL |
50.99 |
50.55 |
-0.44 |
| 2 |
Dunne, Patrick J (20)
|
STTXLA |
51.33 |
50.82 |
-0.51 |
| 3 |
Garnier, Jeffrey B (21)
|
PCSTAN |
51.05 |
50.98 |
-0.07 |
| 4 |
D'Alessandro, Teo A (21)
|
FLBSS |
51.00 |
51.01 |
0.01 |
| 5 |
Cohen, Alexander W (22)
|
GARAYS |
51.05 |
51.05 |
0.00 |
| 6 |
McCormick, Jason P (21)
|
FLNOLE |
51.26 |
51.10 |
-0.16 |
| 7 |
Crosthwaite, Scott T (21)
|
KYUKY |
52.25 |
51.23 |
-1.02 |
| 8 |
Stack, Ryan M (19)
|
WIWA |
51.31 |
51.41 |
0.10 |
| 9 |
Conaton, Patrick A (19)
|
MRBAD |
53.00 |
51.46 |
-1.54 |
| 10 |
Lemaistre, Frederick I (21)
|
STTXLA |
52.00 |
51.48 |
-0.52 |
| 11 |
Duggan, Sean M (21)
|
PCSTAN |
51.05 |
51.58 |
0.53 |
| 12 |
Liang, Andrew W (19)
|
PCSTAN |
51.55 |
51.78 |
0.23 |
| 13 |
Booth, Joshua J (19)
|
SEAU |
51.37 |
51.88 |
0.51 |
| 14 |
Siokos, Konstantinos (27)
|
SCUN-0 |
51.65 |
52.00 |
0.35 |
| 15 |
Kaisrlik, Justin E (21)
|
FLNTCA |
53.00 |
52.07 |
-0.93 |
| 16 |
Trimmer, Luke T (23)
|
INBA |
51.91 |
52.08 |
0.17 |
| 17 |
Lytvenok, Iegor (21)
|
AZGCA |
51.58 |
52.18 |
0.60 |
| 18 |
Love, Dillon H (23)
|
MVUMIZ |
53.00 |
52.19 |
-0.81 |
| 19 |
Molloy, Zachary J (19)
|
NJOCY |
50.96 |
52.25 |
1.29 |
| 20 |
Brown, Cameron (18)
|
USNC |
52.20 |
52.60 |
0.40 |
| 21 |
Cole, Charles M (20)
|
ILWILD |
52.23 |
52.69 |
0.46 |
| 22 |
Keen, Aidan P (17)
|
IAIFLY |
52.36 |
52.95 |
0.59 |
| 23 |
Vitabile, Scott M (19)
|
NJPAA |
52.68 |
53.00 |
0.32 |
| 24 |
Flowers, Braxton A (17)
|
GUCFSC |
52.44 |
53.42 |
0.98 |