| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Clifton, Cannon J (20)
|
WIWA |
50.02 |
50.23 |
0.21 |
| 2 |
Disney-May, James H (23)
|
USGBR |
49.95 |
50.46 |
0.51 |
| 3 |
Peek, James H (20)
|
NCDU |
51.30 |
50.50 |
-0.80 |
| 4 |
Richmond, Renny W (20)
|
AZFORD |
51.59 |
50.70 |
-0.89 |
| 5 |
Barsanti, Ryan J (20)
|
WIWA |
51.00 |
50.75 |
-0.25 |
| 6 |
Stasiunas, Matthew C (20)
|
MAUN-0 |
50.83 |
50.81 |
-0.02 |
| 6 |
Robrock, Kyle J (21)
|
COTOPS |
50.90 |
50.81 |
-0.09 |
| 8 |
Black, Connor S (20)
|
PCSTAN |
50.80 |
50.87 |
0.07 |
| 9 |
Romany, Joshua D (19)
|
SETNAQ |
50.83 |
51.40 |
0.57 |
| 10 |
McCoy, Ryan T (19)
|
AZPSC |
51.13 |
51.70 |
0.57 |
| 11 |
Anderson, Brian M (26)
|
ILUN-0 |
51.30 |
51.81 |
0.51 |
| 12 |
Irwin, Max M (20)
|
INIU |
51.51 |
52.24 |
0.73 |
| 13 |
Asuaje Solon, Julian J (19)
|
NEBGSC |
53.41 |
53.01 |
-0.40 |