| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Conners, Cameron A (23)
|
SEHSA |
1:03.04 |
1:03.01 |
-0.03 |
| 2 |
Eriksson, Niclas E (22)
|
NCQU |
1:01.80 |
1:03.36 |
1.56 |
| 3 |
Alexandrov, Mihail P (31)
|
MRNYAC |
1:00.90 |
1:03.60 |
2.70 |
| 4 |
Mathews, Jason S (17)
|
OHOSSC |
1:03.64 |
1:04.09 |
0.45 |
| 5 |
Whittle, Matthew D (20)
|
SNDART |
1:04.00 |
1:04.34 |
0.34 |
| 6 |
O'Donnell, Jeremiah J (22)
|
VATRIB |
1:03.35 |
1:04.64 |
1.29 |
| 7 |
Yang, Calvin (16)
|
NECRIM |
1:04.41 |
1:04.83 |
0.42 |
| 8 |
Foster, Jacob R (15)
|
OHRAYS |
1:04.11 |
1:04.94 |
0.83 |
| 9 |
Spencer, Maxwell A (23)
|
WVWVU |
1:04.40 |
1:05.10 |
0.70 |
| 10 |
Bornstein, Andrew J (16)
|
CTRAC |
1:04.69 |
1:05.21 |
0.52 |
| 11 |
Wong, Stanley (22)
|
MRNYSA |
1:05.19 |
1:06.84 |
1.65 |
| 12 |
Huang, Scott (20)
|
NIUBST |
1:03.91 |
1:07.03 |
3.12 |