| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Chadwick, Michael H (21)
|
MVUMIZ |
|
48.96 |
- |
| 2 |
Pechmann, Luke A (26)
|
CACANY |
51.76 |
50.51 |
-1.25 |
| 3 |
Szekely, Christopher L (21)
|
MRARMY |
50.72 |
50.60 |
-0.12 |
| 4 |
Peterson, Christopher V (26)
|
PCOAPB |
51.80 |
51.07 |
-0.73 |
| 5 |
Freeman, Robert P (16)
|
SEBAY |
52.50 |
51.90 |
-0.60 |
| 6 |
Kline, Samuel G (18)
|
NTTFA |
52.00 |
52.51 |
0.51 |
| 7 |
Slaton, Micah L (18)
|
STNTRO |
53.23 |
52.64 |
-0.59 |
| 8 |
Erlenmeyer, Daniel J (18)
|
FLSYS |
53.89 |
53.60 |
-0.29 |
|
Dillinger, Thomas J (19)
|
CTGYWD |
51.39 |
NS |
- |
|
Ross, Jordan A (19)
|
GASA |
51.97 |
NS |
- |
|
Cooper, James G (23)
|
GUTWST |
50.72 |
NS |
- |