| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Anderson, Julia A (20)
|
PCSTAN |
57.02 |
56.55 |
-0.47 |
| 2 |
Postoll, Rebecca A (17)
|
NCMAC |
57.03 |
57.17 |
0.14 |
| 3 |
Kuehl, Courtney A (28)
|
AZFORD |
57.39 |
57.35 |
-0.04 |
| 4 |
Deloof, Alexandra M (19)
|
MICW |
56.97 |
57.36 |
0.39 |
| 5 |
Tamblyn, Anne E (20)
|
WIWA |
57.27 |
57.42 |
0.15 |
| 6 |
Sinatro, Haley M (20)
|
MAUN-0 |
58.31 |
57.55 |
-0.76 |
| 7 |
Cox, Megan S (20)
|
LATAQ |
58.50 |
57.64 |
-0.86 |
| 8 |
Mulquin, Catherine E (19)
|
INUND |
57.87 |
57.88 |
0.01 |
| 9 |
Wicks, Samantha J (20)
|
SRBY |
57.60 |
58.04 |
0.44 |
| 10 |
Ontjes, Olivia C (17)
|
NCYOTA |
57.89 |
58.18 |
0.29 |
| 11 |
Yambor-Maul, Alyssa M (19)
|
FLATAC |
59.65 |
58.28 |
-1.37 |
| 12 |
Shu, Ingrid (16)
|
NJLHY |
|
58.58 |
- |
| 13 |
Stoehr, Rachel E (20)
|
ARUARK |
58.00 |
58.67 |
0.67 |
| 14 |
Coker, Tiare U (15)
|
CAROSE |
58.01 |
58.69 |
0.68 |
| 15 |
Lincoln, Hannah E (20)
|
NCMOR |
56.80 |
1:03.02 |
6.22 |
| 16 |
Braun, Elizabeth A (21)
|
ARUARK |
1:03.00 |
1:12.09 |
9.09 |