| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Berg, Madeline M (13)
|
AMSFAC |
56.52 |
57.52 |
1.00 |
| 2 |
Munoz, Kirsten D (13)
|
AMSFAC |
58.92 |
59.25 |
0.33 |
| 3 |
Hull, Marideth G (13)
|
AMUN |
1:01.11 |
59.69 |
-1.42 |
| 4 |
Pietropaolo, Sophia G (13)
|
AMPRA |
1:00.78 |
1:00.84 |
0.06 |
| 5 |
Cain, Eleana E (14)
|
AMUN |
1:02.59 |
1:01.00 |
-1.59 |
| 6 |
Schomaker, Anna J (13)
|
AMPRA |
1:02.00 |
1:01.15 |
-0.85 |
| 7 |
Gongas, Alexandra C (13)
|
AMPRA |
1:06.20 |
1:02.28 |
-3.92 |
| 8 |
Getty, Margaret A (13)
|
AMUN |
1:06.60 |
1:04.75 |
-1.85 |
| 9 |
Gregorowicz, Abigail S (13)
|
AMPRA |
1:06.53 |
1:05.09 |
-1.44 |
| 10 |
Mariner, Ava B (14)
|
AMPTSC |
1:05.72 |
1:05.72 |
0.00 |
| 11 |
Dietiker, Jasmine F (14)
|
AMPTSC |
1:08.59 |
1:07.87 |
-0.72 |
| 12 |
Uzelac, Jillian L (13)
|
AMRXA |
1:11.28 |
1:10.43 |
-0.85 |
| 13 |
Conway, Mackenzie M (13)
|
AMBPR |
1:17.56 |
1:15.31 |
-2.25 |
| 14 |
Sabanovic, Ella (13)
|
AMBPR |
1:19.02 |
1:16.42 |
-2.60 |
| 15 |
Brilhart, Lauren K (13)
|
AMUN |
1:25.15 |
1:25.34 |
0.19 |
| 16 |
Koutavas, Sophia M (14)
|
AMSFAC |
1:29.83 |
1:25.68 |
-4.15 |
| 17 |
Prasanth Reeja, Gourisree (13)
|
AMSFAC |
1:32.77 |
1:29.00 |
-3.77 |