| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rowland, Natalie E (13)
|
AMBWA |
32.28 |
29.63 |
-2.65 |
| 2 |
Gongas, Alexandra C (13)
|
AMPRA |
30.90 |
30.19 |
-0.71 |
| 3 |
Hamilton, Ava M (13)
|
AMUN |
32.54 |
30.49 |
-2.05 |
| 4 |
Zanella, Torri R (13)
|
AMWAAC |
31.06 |
30.97 |
-0.09 |
| 5 |
Droschak, Madison E (13)
|
AMSHAQ |
30.88 |
31.76 |
0.88 |
| 6 |
Pryke, Abigail E (13)
|
AMBWA |
34.84 |
32.25 |
-2.59 |
| 7 |
Peraza, Madison M (13)
|
AMPTSC |
30.73 |
32.47 |
1.74 |
| 8 |
Alexander, Gabrielle C (13)
|
AMSVSC |
31.00 |
32.69 |
1.69 |
| 9 |
Abraham, Elizabeth S (14)
|
AMWAAC |
34.74 |
36.28 |
1.54 |
| 10 |
Wilson, Suzy G (14)
|
AMUN |
39.63 |
36.65 |
-2.98 |
| 11 |
Brilhart, Lauren K (13)
|
AMUN |
36.06 |
37.21 |
1.15 |
| 12 |
Prasanth Reeja, Gourisree (13)
|
AMSFAC |
40.14 |
40.28 |
0.14 |
| 13 |
Koutavas, Sophia M (13)
|
AMSFAC |
39.26 |
40.79 |
1.53 |
|
Albertson, Jessica A (13)
|
AMPRA |
30.43 |
NS |
- |
|
Ramanathan, Mira S (13)
|
AMPRA |
32.92 |
NS |
- |
|
Walko, Paige M (14)
|
AMSVSC |
31.08 |
NS |
- |
|
Chahande, Vidhisha (13)
|
AMSFAC |
36.57 |
NS |
- |
|
Stone, Jane P (13)
|
AMKW |
33.74 |
NS |
- |