| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kolessar, Kaitlyn R (9)
|
MASSC |
41.86 |
39.10 |
-2.76 |
| 2 |
Bookwalter, Olivia M (10)
|
MASSC |
31.61 |
39.50 |
7.89 |
| 3 |
Spinosa, Lindsay C (10)
|
MASSC |
|
40.59 |
- |
| 4 |
Cappola, Alexandra G (10)
|
MASSC |
40.91 |
41.05 |
0.14 |
| 5 |
Kelly, Kathryn J (10)
|
MASSC |
44.47 |
42.44 |
-2.03 |
| 6 |
Kuttymartin, Mira (10)
|
MASSC |
40.84 |
43.44 |
2.60 |
| 7 |
Ott, Cassidy M (9)
|
MASSC |
45.19 |
43.49 |
-1.70 |
| 8 |
Walker, Sofia A (9)
|
MASSC |
|
44.97 |
- |
| 9 |
Slootmaker, Annika L (9)
|
MASSC |
59.80 |
46.83 |
-12.97 |
| 10 |
si, Christina K (9)
|
MASSC |
47.68 |
47.71 |
0.03 |
| 11 |
Watson, Emma G (9)
|
MASSC |
|
48.84 |
- |
| 12 |
Rahardjo, Seraphim C (9)
|
MASSC |
|
49.17 |
- |
| 13 |
Berlanska, Anya S (9)
|
MASSC |
|
52.31 |
- |
| 14 |
Wanner, Sydney L (9)
|
MAUNAT |
|
57.92 |
- |
| 15 |
Kelly, Theresa R (9)
|
MASSC |
|
1:03.66 |
- |