| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kolessar, Ashley O (12)
|
MASSC |
27.07 |
25.71 |
-1.36 |
| 2 |
Feuzeu Mekoue, Laurielle Kel (12)
|
MASSC |
26.70 |
26.15 |
-0.55 |
| 3 |
Bergstrom, Sydney L (12)
|
MASSC |
26.17 |
26.38 |
0.21 |
| 4 |
Mullins, Lainey J (11)
|
MASSC |
26.36 |
26.58 |
0.22 |
| 5 |
Eruslanova, Ekaterina (11)
|
MASSC |
28.60 |
27.19 |
-1.41 |
| 6 |
Lunn, Emma K (11)
|
MASSC |
26.27 |
27.47 |
1.20 |
| 7 |
Paglia, Sophia B (12)
|
MASSC |
30.50 |
29.09 |
-1.41 |
| 8 |
Holtsberg, Cathryn F (12)
|
MASSC |
29.31 |
29.11 |
-0.20 |
| 9 |
Levensten, Kate E (11)
|
MASSC |
30.36 |
29.25 |
-1.11 |
| 10 |
Whitaker, Kallie V (12)
|
MASSC |
28.48 |
29.43 |
0.95 |
| 11 |
Levensten, Sydney J (11)
|
MASSC |
31.74 |
29.47 |
-2.27 |
| 12 |
Johnson, Anna K (11)
|
MASSC |
30.53 |
30.74 |
0.21 |
| 13 |
Shifflett, Ella M (12)
|
MASSC |
31.06 |
30.96 |
-0.10 |
| 14 |
Impriano, Madilyn G (11)
|
MASSC |
|
34.82 |
- |
| 15 |
Murnaghan, Ella A (12)
|
MASSC |
38.55 |
36.54 |
-2.01 |
| 16 |
Rayer, Anne-Aurora (12)
|
MASSC |
41.77 |
36.89 |
-4.88 |
| 17 |
Haub, Ava E (11)
|
MASSC |
|
43.68 |
- |
| 18 |
Callahan, Jessica A (11)
|
MASSC |
47.42 |
44.18 |
-3.24 |
|
Wells, De'Jah (11)
|
MASSC |
28.92 |
NS |
- |