| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Diguey, Velina (10)
|
MASSC |
42.03 |
41.27 |
-0.76 |
| 2 |
Kolessar, Kaitlyn R (9)
|
MASSC |
46.30 |
43.59 |
-2.71 |
| 3 |
Gordon, Grace C (10)
|
MASSC |
50.32 |
45.44 |
-4.88 |
| 4 |
Mitchell, Victoria S (10)
|
MASSC |
45.81 |
45.92 |
0.11 |
| 5 |
Kuttymartin, Mira (10)
|
MASSC |
49.05 |
46.91 |
-2.14 |
| 6 |
Foppa, Ashley (9)
|
MASSC |
|
49.24 |
- |
| 7 |
Cappola, Alexandra G (10)
|
MASSC |
48.11 |
51.64 |
3.53 |
| 8 |
Spinosa, Lindsay C (10)
|
MASSC |
|
51.94 |
- |
| 9 |
Walker, Sofia A (9)
|
MASSC |
55.17 |
52.23 |
-2.94 |
| 10 |
Berlanska, Anya S (9)
|
MASSC |
|
57.92 |
- |
| 11 |
Rahardjo, Seraphim C (9)
|
MASSC |
|
59.83 |
- |
| 12 |
Slootmaker, Annika L (9)
|
MASSC |
1:03.39 |
1:00.71 |
-2.68 |
|
Hurley, Mia B (10)
|
MASSC |
|
DQ |
- |