| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kolessar, Ashley O (12)
|
MASSC |
35.23 |
34.59 |
-0.64 |
| 2 |
Feuzeu Mekoue, Laurielle Kel (12)
|
MASSC |
34.26 |
35.30 |
1.04 |
| 3 |
Holtsberg, Cathryn F (12)
|
MASSC |
36.70 |
35.71 |
-0.99 |
| 4 |
Bergstrom, Sydney L (12)
|
MASSC |
34.79 |
35.78 |
0.99 |
| 5 |
Eruslanova, Ekaterina (11)
|
MASSC |
37.31 |
36.79 |
-0.52 |
| 6 |
Levensten, Kate E (11)
|
MASSC |
41.97 |
39.54 |
-2.43 |
| 7 |
Levensten, Sydney J (11)
|
MASSC |
42.62 |
40.93 |
-1.69 |
| 8 |
Paglia, Sophia B (12)
|
MASSC |
41.95 |
40.94 |
-1.01 |
| 9 |
Whitaker, Kallie V (12)
|
MASSC |
43.11 |
41.35 |
-1.76 |
| 10 |
Krieg, Sylvia C (11)
|
MASSC |
44.17 |
42.81 |
-1.36 |
| 11 |
Rayer, Anne-Aurora (12)
|
MASSC |
50.23 |
44.98 |
-5.25 |
| 12 |
Impriano, Madilyn G (11)
|
MASSC |
1:07.62 |
45.42 |
-22.20 |
| 13 |
Murnaghan, Ella A (12)
|
MASSC |
52.30 |
50.38 |
-1.92 |
| 14 |
Callahan, Jessica A (11)
|
MASSC |
1:01.39 |
1:02.07 |
0.68 |