| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Getty, Margaret A (13)
|
AMUN |
2:40.94 |
2:40.12 |
-0.82 |
| 2 |
Dietiker, Jasmine F (14)
|
AMPTSC |
2:53.03 |
2:50.31 |
-2.72 |
| 3 |
Maxin, Rebecca D (14)
|
AMSFAC |
2:47.39 |
2:51.51 |
4.12 |
| 4 |
Newbould, Grace A (13)
|
AMBWA |
3:00.75 |
2:53.70 |
-7.05 |
| 5 |
Gongas, Alexandra C (13)
|
AMPRA |
3:07.72 |
2:56.30 |
-11.42 |
| 6 |
Mariner, Ava B (13)
|
AMPTSC |
2:50.15 |
2:56.62 |
6.47 |
| 7 |
Stier, Alycia M (13)
|
AMSFAC |
3:02.75 |
2:56.63 |
-6.12 |
| 8 |
Chen, Ally Y (13)
|
AMPTSC |
2:56.13 |
2:58.58 |
2.45 |
| 9 |
Butler, Aurora T (13)
|
AMWAAC |
3:28.15 |
3:08.91 |
-19.24 |
| 10 |
Zanella, Torri R (13)
|
AMWAAC |
3:13.69 |
3:10.10 |
-3.59 |
| 11 |
Mosca, Gianna R (13)
|
AMUN |
3:00.84 |
3:11.72 |
10.88 |
| 12 |
Deybiswas, Juhi (13)
|
AMSFAC |
4:20.73 |
3:40.32 |
-40.41 |
| 13 |
Prasanth Reeja, Gourisree (13)
|
AMSFAC |
3:47.19 |
3:46.80 |
-0.39 |
| 14 |
Koutavas, Sophia M (13)
|
AMSFAC |
3:46.17 |
3:53.31 |
7.14 |
|
Deybiswas, Jasmine I (13)
|
AMSFAC |
3:53.68 |
DQ |
- |
|
Chahande, Vidhisha (13)
|
AMSFAC |
3:30.60 |
NS |
- |
|
Walko, Paige M (14)
|
AMSVSC |
2:58.90 |
NS |
- |
|
Ramanathan, Mira S (13)
|
AMPRA |
3:01.51 |
NS |
- |
|
Albertson, Jessica A (13)
|
AMPRA |
3:02.68 |
NS |
- |