| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
O'Shaughnessy, Mary M (12)
|
PVFISH |
38.90 |
39.94 |
1.04 |
| 2 |
Kuang, Gloria J (11)
|
PVDRAG |
40.00 |
41.22 |
1.22 |
| 3 |
Cason, Ameenah B (12)
|
MDBCSC |
42.97 |
43.70 |
0.73 |
| 4 |
Keggins, Peyton L (11)
|
GUPACK |
46.15 |
45.32 |
-0.83 |
| 5 |
Sumpter, Emerson A (11)
|
MDBCSC |
42.65 |
46.44 |
3.79 |
| 6 |
Lu, Emma L (12)
|
MDCAA |
46.07 |
47.40 |
1.33 |
| 7 |
Woodley, Abree J (12)
|
MDBCSC |
45.72 |
47.88 |
2.16 |
| 8 |
Cahill, Kimberly R (12)
|
MDBCSC |
47.85 |
48.21 |
0.36 |
| 9 |
Grantland, Kara M (12)
|
MDSPRC |
46.79 |
48.37 |
1.58 |
| 10 |
Chatradi, Sirihamsini (12)
|
PVDRAG |
47.06 |
48.81 |
1.75 |
| 11 |
Simmons, Emily K (11)
|
MDBAAC |
49.21 |
48.98 |
-0.23 |
| 12 |
Walsh, Aivleen E (11)
|
MAYY |
55.15 |
49.55 |
-5.60 |
| 13 |
Deshpande, Ananya U (12)
|
MDRAC |
|
50.11 |
- |
| 14 |
St. Pierre, Deanna R (12)
|
MDJCC |
55.96 |
50.32 |
-5.64 |
| 15 |
Rios, Abbyflorianne G (11)
|
MDBAAC |
55.43 |
50.83 |
-4.60 |
| 16 |
Chen, Rayna L (11)
|
MDBAAC |
51.99 |
50.96 |
-1.03 |
| 17 |
Dumesh, Ariela M (11)
|
MDRAC |
50.33 |
51.86 |
1.53 |
| 18 |
Ross, Shanyia R (12)
|
MDBCSC |
|
52.18 |
- |
| 19 |
Weber, Leila A (11)
|
MDRAC |
|
54.25 |
- |
| 20 |
Ellenbecker, Vivienne N (11)
|
MDBAAC |
1:03.92 |
54.40 |
-9.52 |
| 21 |
Krownapple, Josephine M (11)
|
MDCAA |
55.87 |
56.21 |
0.34 |
| 22 |
Johnson, Kaitlyn (12)
|
MASAKA |
|
57.13 |
- |
| 23 |
Zayas, Jubelees (12)
|
MDBCSC |
55.15 |
58.09 |
2.94 |
| 24 |
Curry, McKinley A (11)
|
MDBCSC |
1:02.48 |
1:01.87 |
-0.61 |
| 25 |
Johnson, Maleah A (11)
|
MDBCSC |
|
1:11.04 |
- |
| 26 |
Toomer, Heaven I (11)
|
MDBCSC |
|
1:22.71 |
- |
|
Williamson, Kaiya C (12)
|
MDBCSC |
|
DQ |
- |
|
Bolden, Jaelyn R (12)
|
MDBCSC |
|
DQ |
- |