| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Collins, Daisy B (13)
|
NCNCAC |
55.22 |
55.29 |
0.07 |
| 2 |
Thornburg, Sophie J (14)
|
NCNCAC |
56.34 |
58.59 |
2.25 |
| 3 |
Kim, Kenzie A (14)
|
NCNCAC |
56.84 |
59.72 |
2.88 |
| 4 |
Collins, Mackenzie K (14)
|
NCNCAC |
54.96 |
59.79 |
4.83 |
| 5 |
Burleigh, Sydney R (14)
|
NCNCAC |
59.60 |
1:00.28 |
0.68 |
| 6 |
Hill, Eleanor E (14)
|
NCNCAC |
56.55 |
1:00.50 |
3.95 |
| 7 |
Chen, Guanyan D (13)
|
NCNCAC |
1:00.03 |
1:02.11 |
2.08 |
| 8 |
Meiferdt, Natalie S (14)
|
NCNCAC |
1:00.13 |
1:02.64 |
2.51 |
| 9 |
Hilton, Avery N (13)
|
NCNCAC |
1:03.27 |
1:03.94 |
0.67 |
| 10 |
Yao, Cana (14)
|
NCNCAC |
1:02.41 |
1:04.35 |
1.94 |
| 11 |
Haire, Sydney M (14)
|
NCNCAC |
1:01.46 |
1:04.80 |
3.34 |
| 12 |
Gallo, Helen N (13)
|
NCNCAC |
1:06.12 |
1:05.31 |
-0.81 |
| 13 |
Li, Joie N (14)
|
NCNCAC |
1:07.53 |
1:07.08 |
-0.45 |
| 14 |
Burton, Chloe G (14)
|
NCNCAC |
1:04.45 |
1:07.92 |
3.47 |
| 15 |
Biswas, Emma A (13)
|
NCNCAC |
1:05.84 |
1:08.00 |
2.16 |
| 16 |
Mangum, Kaylee A (14)
|
NCUN-0 |
1:12.20 |
1:10.18 |
-2.02 |
| 17 |
Brown, Jade T (14)
|
NCNCAC |
1:07.74 |
1:11.92 |
4.18 |
| 18 |
Benesch, Marta I (13)
|
NCNCAC |
1:22.41 |
1:15.24 |
-7.17 |
| 19 |
Wolter, Adelyn M (13)
|
NCNCAC |
1:25.99 |
1:16.26 |
-9.73 |
| 20 |
Goodnight, Caroline R (14)
|
NCNCAC |
1:09.37 |
1:16.57 |
7.20 |
| 21 |
Haire, Juliette N (13)
|
NCNCAC |
1:16.39 |
1:19.94 |
3.55 |