| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wolk, Isabel H (16)
|
NCBLUE |
57.77 |
59.40 |
1.63 |
| 2 |
Emrich, Abigail G (16)
|
NCBLUE |
58.94 |
1:01.57 |
2.63 |
| 3 |
Burleigh, Sydney R (15)
|
NCWHIT |
58.73 |
1:02.22 |
3.49 |
| 4 |
Wolk, Audrey J (17)
|
NCWHIT |
58.83 |
1:02.60 |
3.77 |
| 5 |
Hill, Eleanor E (15)
|
NCBLUE |
1:02.14 |
1:04.22 |
2.08 |
| 6 |
Collins, Mackenzie K (15)
|
NCBLUE |
1:00.42 |
1:04.42 |
4.00 |
| 7 |
Mihaly, Chloe C (16)
|
NCWHIT |
1:01.08 |
1:04.60 |
3.52 |
| 8 |
Thornburg, Sophie J (15)
|
NCBLUE |
1:02.44 |
1:06.12 |
3.68 |
| 9 |
Kim, Kenzie A (15)
|
NCWHIT |
1:05.14 |
1:06.74 |
1.60 |
| 10 |
Shuping, Avery E (16)
|
NCBLUE |
1:01.30 |
1:06.82 |
5.52 |
| 11 |
Dehring, Natalie M (17)
|
NCBLUE |
1:07.98 |
1:08.62 |
0.64 |
| 12 |
Dolan, Maya A (17)
|
NCBLUE |
1:00.87 |
1:08.70 |
7.83 |
| 13 |
Biggerstaff, Jordan R (16)
|
NCWHIT |
1:07.20 |
1:10.04 |
2.84 |
| 14 |
Guaman, Evelyn S (16)
|
NCWHIT |
1:08.19 |
1:14.30 |
6.11 |
| 15 |
Reed, Nancy H (16)
|
NCBLUE |
1:16.56 |
1:21.99 |
5.43 |
|
Brown, Avery R (17)
|
NCWHIT |
1:03.82 |
DQ |
- |