| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Midura, Katelin M (12)
|
MAJRAY |
38.04 |
38.03 |
-0.01 |
| 1 |
DeCardona, Ella G (12)
|
MAGAAC |
42.62 |
42.66 |
0.04 |
| 2 |
Steinmetz, Zoe N (11)
|
MANSC |
39.22 |
38.08 |
-1.14 |
| 2 |
McCrudden, Reiley M (12)
|
MAGAAC |
|
43.02 |
- |
| 3 |
Meyers, Isabella M (12)
|
MANSC |
38.60 |
38.35 |
-0.25 |
| 3 |
Macturk, Jeannette L (12)
|
MAWCAC |
46.28 |
44.48 |
-1.80 |
| 4 |
Henriquez, Nicole A (12)
|
MANSC |
45.71 |
45.21 |
-0.50 |
| 5 |
Rasmussen, Rebecca S (11)
|
MAFSS |
48.12 |
47.56 |
-0.56 |
| 6 |
Ali, Ayan A (11)
|
MASERP |
59.49 |
52.62 |
-6.87 |
| 7 |
Bhatia, Saanvi (12)
|
MAWCAC |
54.22 |
55.96 |
1.74 |
| 8 |
Zaker, Maimoonah K (11)
|
MASERP |
58.11 |
57.29 |
-0.82 |
| 9 |
Schwab, Mary Ruth M (12)
|
MASERP |
1:15.69 |
1:14.13 |
-1.56 |