| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Esposito, William P (16)
|
NCGCY |
28.94 |
29.29 |
0.35 |
| 2 |
Zhang, Ray (16)
|
NCGCY |
31.70 |
30.56 |
-1.14 |
| 3 |
Gatling III, Ladaniel (15)
|
NCGCY |
33.39 |
30.98 |
-2.41 |
| 4 |
Jerger, Jacob A (16)
|
NCWA |
41.82 |
31.80 |
-10.02 |
| 5 |
Yeh, Ryan T (16)
|
NCLOY |
34.12 |
32.56 |
-1.56 |
| 6 |
Yeh, Alexander T (15)
|
NCLOY |
35.60 |
32.69 |
-2.91 |
| 7 |
Carsten, Nathaniel D (16)
|
NCLOY |
32.73 |
32.84 |
0.11 |
| 8 |
Muller, Nicholas A (17)
|
NCLOY |
34.53 |
33.07 |
-1.46 |
| 9 |
Vagner, Braxton S (16)
|
NCRACY |
|
33.62 |
- |
| 10 |
Lucktong, Samuel P (15)
|
NCLOY |
36.74 |
33.93 |
-2.81 |
| 11 |
Cao, Benjamin M (15)
|
NCLOY |
40.78 |
34.52 |
-6.26 |
| 12 |
Conde, Anthony J (16)
|
NCLOY |
36.56 |
34.97 |
-1.59 |
| 13 |
Beck, Zachary D (16)
|
NCLOY |
40.19 |
35.84 |
-4.35 |
| 14 |
Claborn, Nathan M (15)
|
NCLOY |
39.02 |
37.13 |
-1.89 |
| 15 |
Krafft, Jordan L (15)
|
NCWA |
|
39.84 |
- |
|
Latorre, Daniel S (16)
|
NCLOY |
|
NS |
- |