| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Holmes, Jeremiah J (17)
|
KYAAY |
25.00 |
24.60 |
-0.40 |
| 2 |
Risma, Sammy I (16)
|
OHBASH |
25.14 |
25.42 |
0.28 |
| 3 |
Holmes, Calyx J (15)
|
KYAAY |
26.24 |
25.56 |
-0.68 |
| 4 |
Valentine, Isaiah M (17)
|
OHPCY |
26.31 |
25.92 |
-0.39 |
| 5 |
Tarquin, James P (18)
|
FLYCF |
25.85 |
25.94 |
0.09 |
| 6 |
Grimmett, Bennett B (18)
|
VALY |
25.67 |
25.97 |
0.30 |
| 7 |
Van der Linden, Brandon E (17)
|
NCGCY |
25.41 |
26.04 |
0.63 |
| 8 |
Sadler, Noah P (16)
|
FLYCF |
26.69 |
26.08 |
-0.61 |
| 9 |
Fischer, Nikolas E (17)
|
ILBRRY |
25.49 |
26.20 |
0.71 |
| 10 |
Quint, Logan M (20)
|
FLYCF |
26.10 |
26.21 |
0.11 |
| 11 |
Lorenz, Michael A (16)
|
OHPCY |
27.12 |
26.26 |
-0.86 |
| 12 |
Doromal, James P (15)
|
ILBRRY |
27.05 |
26.30 |
-0.75 |
| 13 |
Enderle, Benjamin L (18)
|
OHPCY |
27.64 |
26.41 |
-1.23 |
| 14 |
Womble, Ethan V (15)
|
NCGCY |
27.16 |
26.54 |
-0.62 |
| 14 |
Brocker, Philip G (17)
|
OHPCY |
25.66 |
26.54 |
0.88 |
| 16 |
Murray, Ethan Y (16)
|
CTWYW |
|
26.77 |
- |
| 17 |
Kalahiki, Khalif-Munir I (17)
|
FLYCF |
28.38 |
26.85 |
-1.53 |
| 18 |
Sherman, Adam J (16)
|
OHPCY |
28.19 |
27.03 |
-1.16 |
| 19 |
Nader, Daniel M (17)
|
OHPCY |
28.71 |
27.04 |
-1.67 |
| 20 |
Gatling III, Ladaniel (14)
|
NCGCY |
27.74 |
27.39 |
-0.35 |
| 21 |
Prophater, Robert L (18)
|
OHPCY |
26.66 |
27.74 |
1.08 |
| 22 |
Esposito, William P (15)
|
NCGCY |
29.24 |
29.15 |
-0.09 |
|
Jordan, William D (18)
|
OHPCY |
28.90 |
DQ |
- |
|
Johnson, David K (15)
|
OHPCY |
27.89 |
DQ |
- |
|
Conley, Brendan M (16)
|
OHABLY |
26.13 |
NS |
- |