| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Knightly, Peyton D (12)
|
NECRIM |
|
5:22.17 |
- |
| 2 |
Braun, Gretchen L (10)
|
ADNTS |
5:35.54 |
5:22.58 |
-12.96 |
| 3 |
Denton, Meghan J (12)
|
NEGMA |
5:49.67 |
5:43.07 |
-6.60 |
| 4 |
Sellnow, Abigail V (12)
|
ADEELS |
6:24.66 |
5:44.97 |
-39.69 |
| 5 |
Boenau, Carlye Ana T (11)
|
ADBFIN |
5:51.19 |
5:50.84 |
-0.35 |
| 6 |
Bard, Alexandria S (12)
|
ADEELS |
6:24.78 |
5:51.19 |
-33.59 |
| 7 |
O'Donnell, Maura E (11)
|
ADEELS |
6:24.83 |
5:59.23 |
-25.60 |
| 8 |
Harris, Sierra A (12)
|
NEGMA |
6:11.66 |
6:03.56 |
-8.10 |
| 9 |
Wright, Becca M (11)
|
ADEELS |
|
6:06.88 |
- |
| 10 |
Stever, Natalia D (12)
|
NEGMA |
6:10.52 |
6:10.33 |
-0.19 |
| 11 |
Fraley, Kaiya R (11)
|
ADBFIN |
|
6:11.24 |
- |
| 12 |
Mienaltowski, Allison E (12)
|
ADEELS |
6:13.90 |
6:13.32 |
-0.58 |
| 13 |
Sanderson, Skyler G (10)
|
ADNTS |
6:52.74 |
6:23.86 |
-28.88 |
| 14 |
Sellnow, Emma M (11)
|
ADEELS |
|
6:36.64 |
- |
| 15 |
Fiacco, Briella P (10)
|
ADBFIN |
|
6:43.81 |
- |
| 16 |
Fraley, Ella (9)
|
ADBFIN |
|
6:58.51 |
- |
| 17 |
Kropp, Paige H (10)
|
ADEELS |
|
7:07.97 |
- |
| 18 |
Thompson, Hailey M (11)
|
ADNTS |
7:47.02 |
7:16.53 |
-30.49 |