| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Waugh, Tyler N (10)
|
PVDCPR |
35.31 |
37.36 |
2.05 |
| 2 |
Smith, Virginia H (9)
|
PVDCPR |
40.80 |
40.45 |
-0.35 |
| 3 |
Brinkley-Tyson, Malaijah A (10)
|
PVDCPR |
41.56 |
42.76 |
1.20 |
| 4 |
Asavakarin, Cate (9)
|
PVLIFE |
45.10 |
43.47 |
-1.63 |
| 5 |
Baek, Isabel (9)
|
PVLIFE |
|
44.32 |
- |
| 6 |
Klugman, Ariella M (10)
|
PVLCL |
|
44.45 |
- |
| 7 |
Faustini, Nina M (10)
|
PVLIFE |
46.90 |
44.57 |
-2.33 |
| 8 |
Smith, Aurelia J (10)
|
PVDCPR |
51.04 |
45.09 |
-5.95 |
| 9 |
Nguyen, Alyssa T (9)
|
PVNCAP |
47.46 |
45.20 |
-2.26 |
| 10 |
Burakiewicz, Imogen C (10)
|
PVLCL |
48.32 |
46.83 |
-1.49 |
| 11 |
Proctor, Rainaka R (10)
|
PVDCPR |
46.80 |
48.16 |
1.36 |
| 12 |
Hammer, Sophia G (9)
|
PVPAC |
44.79 |
48.33 |
3.54 |
| 13 |
Turner, Lena S (9)
|
PVNCAP |
45.38 |
50.18 |
4.80 |
| 14 |
Ortiz, Elizabeth (10)
|
PVLIFE |
|
50.52 |
- |
| 15 |
Brooks, Sydney (9)
|
PVHEAL |
51.19 |
50.90 |
-0.29 |
| 16 |
Johnson, Alexa M (10)
|
PVLIFE |
51.45 |
52.03 |
0.58 |
| 17 |
Ferus, Viktoria (9)
|
PVPAC |
56.38 |
53.12 |
-3.26 |
| 18 |
Hutchcroft, Paige F (9)
|
PVPAC |
50.74 |
53.23 |
2.49 |
| 19 |
Parham, Chloe M (9)
|
PVNCAP |
54.15 |
53.33 |
-0.82 |
| 20 |
Coble, Lily I (9)
|
PVPAC |
51.37 |
53.85 |
2.48 |
| 21 |
Ellsworth, Sloan K (9)
|
PVDCPR |
1:03.33 |
54.21 |
-9.12 |
| 22 |
Charles-Balthazar, Danariell (9)
|
PVNCAP |
53.23 |
56.47 |
3.24 |
| 23 |
Wassberg, Sabrina I (9)
|
PVPAC |
58.64 |
56.98 |
-1.66 |
| 24 |
Smith, Molly E (9)
|
PVPAC |
|
57.82 |
- |
| 25 |
Kokkoros, Viviana (9)
|
PVLCL |
|
58.22 |
- |
| 26 |
Curry, Sophie R (9)
|
PVDCPR |
|
1:07.00 |
- |
|
Miller, Ellie L (10)
|
MDACSS |
47.68 |
NS |
- |
|
Mensah, Destinie N (10)
|
PVPAC |
|
NS |
- |
|
Gallup, Kendall A (10)
|
MDACSS |
43.81 |
NS |
- |