| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Espinoza-Soriano, Jorge (14)
|
MRMHHW |
24.88 |
25.43 |
0.55 |
| 2 |
Abdel-Kader, Anthony P (13)
|
MRMWDA |
25.80 |
25.63 |
-0.17 |
| 3 |
Walz, Charles F (13)
|
CTSYS |
27.32 |
26.53 |
-0.79 |
| 4 |
Gaynor, Declan T (13)
|
MRRFAC |
27.27 |
27.26 |
-0.01 |
| 5 |
Croutch, Tyler D (14)
|
MRMWDA |
27.07 |
27.35 |
0.28 |
| 6 |
Howard, Sam W (14)
|
MRRFAC |
28.97 |
28.09 |
-0.88 |
| 7 |
Fisher, Samuel (14)
|
MRDVWM |
29.12 |
28.30 |
-0.82 |
| 8 |
Torres, Jeremy T (14)
|
MRWSSC |
29.02 |
28.67 |
-0.35 |
| 9 |
Perry, Jasper D (13)
|
CTSYS |
29.40 |
29.02 |
-0.38 |
| 10 |
Robinson, Jacob J (13)
|
MRNDAC |
29.79 |
29.09 |
-0.70 |
| 11 |
Raszkiewicz, Alex R (13)
|
MRRFAC |
29.63 |
29.84 |
0.21 |
| 12 |
Martinez, Eztli (13)
|
MRMHHW |
29.89 |
30.13 |
0.24 |
| 13 |
Williamson, Taggart S (13)
|
MRVAC |
31.65 |
31.42 |
-0.23 |
| 14 |
Malvacia, David (13)
|
MRWSSC |
37.99 |
32.04 |
-5.95 |
| 15 |
Oppmann, Daniel G (13)
|
MRMWDA |
32.46 |
32.09 |
-0.37 |
| 16 |
Shaw, Jaden K (14)
|
MRMWDA |
31.95 |
32.93 |
0.98 |
| 17 |
Shauger, Haiden W (14)
|
MRDVWM |
33.24 |
33.34 |
0.10 |
| 18 |
Scalia, Pietro N (13)
|
MRWSSC |
34.78 |
33.84 |
-0.94 |
| 19 |
Kantner, Noah F (13)
|
MRYBAR |
33.14 |
34.55 |
1.41 |
| 20 |
Kilgore, Conor F (14)
|
MRPBAC |
48.78 |
35.42 |
-13.36 |
| 21 |
Feudo, Luke A (13)
|
MRWSSC |
37.99 |
39.16 |
1.17 |