| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Walker, Lucy E (15)
|
MRBGNW |
52.50 |
52.48 |
-0.02 |
| 2 |
Sohn, Sora (17)
|
MRBGNW |
54.36 |
53.44 |
-0.92 |
| 3 |
Whang, Taylor K (17)
|
MRBGNW |
55.01 |
53.98 |
-1.03 |
| 4 |
Paez, Isabella S (16)
|
MRBGNW |
53.37 |
55.71 |
2.34 |
| 5 |
Rocco, Emily A (17)
|
MRBGNW |
54.18 |
55.93 |
1.75 |
| 6 |
Iannetta, Ava L (17)
|
MRBGNW |
53.91 |
57.07 |
3.16 |
| 7 |
Blakeslee, Kaitlin P (17)
|
MRBGNW |
56.62 |
58.01 |
1.39 |
| 8 |
Patterson, Clare M (16)
|
MRBGNW |
57.29 |
58.21 |
0.92 |
| 9 |
Slive, Ellie R (15)
|
MRBGNW |
56.38 |
58.98 |
2.60 |
|
Alexander, Sinceraty D (17)
|
MRBGNW |
54.75 |
NS |
- |
|
Williams, Laura A (15)
|
MRBGNW |
1:04.50 |
NS |
- |