| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Smith, Virginia H (10)
|
PVDCPR |
41.86 |
42.54 |
0.68 |
| 2 |
Schmitt, Hannah (9)
|
PVNCAP |
55.66 |
48.25 |
-7.41 |
| 3 |
Ellsworth, Sloan K (10)
|
PVDCPR |
58.78 |
51.05 |
-7.73 |
| 3 |
Guerrieri Brown, Giulia (10)
|
PVDCPR |
51.98 |
51.05 |
-0.93 |
| 5 |
Pascuccio Gomez, Fiona (10)
|
PVDCPR |
52.64 |
51.15 |
-1.49 |
| 6 |
Ezell, Makayla E (10)
|
PVDCPR |
51.19 |
53.55 |
2.36 |
| 7 |
Walker, Beatrice E (9)
|
PVNCAP |
56.01 |
55.39 |
-0.62 |
| 8 |
Minalu, Kalkidan A (9)
|
PVDCPR |
56.98 |
55.58 |
-1.40 |
| 9 |
Ipakchi, Elina M (9)
|
PVNCAP |
1:01.07 |
57.65 |
-3.42 |
| 10 |
Jefferson, Alexis N (10)
|
PVDCPR |
58.42 |
59.78 |
1.36 |
| 11 |
Dillon, Charlotte T (9)
|
PVDCPR |
|
1:07.18 |
- |